A quadruple-labeling luminescence strategy for multiplexed immunoassay of 51 drugs in milk with an automated pretreatment system was written by Zhang, Yaqing;Chang, Xiaoxi;Wang, Xin;Tao, Xiaoqi. And the article was included in Analytical Methods in 2019.Computed Properties of C19H20F3N3O3 This article mentions the following:
In this study, a novel combination of quadruple-labeling luminescence strategy multiplexed immunoassay (QLL-MIA) and an automated pretreatment system for simultaneous extraction and determination of 20 fluoroquinolones (FQs), 15 β-lactams, 15 sulfonamides (SAs), and chloramphenicol (CAP) in milk was developed. The strategy integrated 2 fluorescence probes, and 2 chemiluminescence probes to achieve signal separation, and a new combination of a nucleic acid extraction system and immunomagnetic beads (IMBs) as the automated pretreatment system to achieve simultaneous determination of 51 drugs. Taking advantage of automated pretreatment system for concentrating milk sample with 5 times and saving the extraction time, the limits of detection (LODs) for FQs, β-lactams, SAs, and CAP in the QLL-MIA range from 29.1 ng L-1 for ciprofloxacin (CIP) to 8000.0 ng L-1 (8.0μg L-1) for trovafloxacin (TRO), 20.0 ng L-1 for ceftiofur (CEF) to 3409.1 ng L-1 (3.4μg L-1) for cephalexin (CEL), 8.6 ng L-1 for sulfadimethoxine (SDM) to 328.2 ng L-1 (0.3μg L-1) for sulfadiazine (SDZ), and 6.0 ng L-1 for CAP. The recoveries ranged from 80.6% to 105.5% at a fortified concentration of LOD and 2 LOD, with a coefficient of variation <15%. Anal. of field milk samples by the combination of QLL-MIA and the automated pretreatment system (the developed QLL-MIA) was in accordance with that of liquid chromatog. coupled with tandem mass spectrometry (LC-MS/MS). The above results demonstrated that the developed QLL-MIA could simultaneously screen trace amounts of FQs, β-lactams, SAs, and CAP in field samples, suitable for high-throughput screening of low-mol. weight contaminants. In the experiment, the researchers used many compounds, for example, 1-Cyclopropyl-7-(cis-3,5-dimethylpiperazin-1-yl)-5,6,8-trifluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (cas: 113617-63-3Computed Properties of C19H20F3N3O3).
1-Cyclopropyl-7-(cis-3,5-dimethylpiperazin-1-yl)-5,6,8-trifluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (cas: 113617-63-3) belongs to piperazine derivatives. Simple N-substituted piperazines have been found in many drug molecules. Piperazine and its salts did not induce point mutations in a bacterial test. A series of mutagenicity studies in cells, both in vitro and in vivo, has been completed and showed no evidence of mutagenic effect.Computed Properties of C19H20F3N3O3
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics