Development and validation of HPLC/DAD method for simultaneously determination of six prohibited substances in model matrices was written by Zaharieva, Zdravka;Tanev, Dimitar;Danalev, Dancho. And the article was included in Acta Chromatographica in 2020.HPLC of Formula: 224785-90-4 This article mentions the following:
The authorities have identified an emerging trend where over-the-counter products, represented as dietary supplements, contain hidden active ingredients that could be harmful. Consumers may unknowingly take products laced with varying quantities of approved prescription drug ingredients, controlled substances, and untested and unstudied pharmaceutically active ingredients. Hidden ingredients are increasingly becoming a problem in products promoted for sexual enhancement, weight loss, or bodybuilding. The tests have revealed the presence of some undesired substances like sildenafil, tadalafil, vardenafil, and their analogs in tainted sexual enhancement products. The content of these substances is usually around the daily curative dose. A simple high-performance liquid chromatog. (HPLC) method for simultaneously determination of sildenafil, vardenafil, tadalafil, dapoxetine, yohimbine, and sibutramine was developed and validated. InfinityLab Poroshell 120 EC-C18 (150 ‘4.6 mm ‘4μm particles) was used, as well as a diode-array detector (DAD) at 230 nm, and a gradient flow with 0.030 M ammonium acetate buffer and acetonitrile. The method is linear in the following range: 2.5-37.5μg/mL for yohimbine, 2.06-30.9μg/ mL for vardenafil, 2.0-30.0μg/mL for sildenafil, 3.1-46.5μg/mL for tadalafil, 1.98-29.7μg/mL for dapoxetine, and 2.2-66.0μg/mL for sibutramine. The linearity coefficient is R2 = 1 for all substances. Model matrixes were spiked, and the anal. recoveries for all substances are in the range 97.5%-99.5%. The method exhibited an upper hand compared with previously reported methods in terms of speed and simplicity. Addnl., the mobile phase (also used as extracting, column washing, and diluting solvent) was composed of only buffer and acetonitrile, which rendered the method much cheaper than others. In the experiment, the researchers used many compounds, for example, 2-(2-Ethoxy-5-((4-ethylpiperazin-1-yl)sulfonyl)phenyl)-5-methyl-7-propylimidazo[5,1-f][1,2,4]triazin-4(3H)-one (cas: 224785-90-4HPLC of Formula: 224785-90-4).
2-(2-Ethoxy-5-((4-ethylpiperazin-1-yl)sulfonyl)phenyl)-5-methyl-7-propylimidazo[5,1-f][1,2,4]triazin-4(3H)-one (cas: 224785-90-4) belongs to piperazine derivatives. Piperazine causes primary dermal irritation and skin burns at high concentrations. Piperazine also causes eye irritation in humans. Although many piperazine derivatives occur naturally, piperazine itself can be synthesized by reacting alcoholic ammonia with 1,2-dichloroethane, by the action of sodium and ethylene glycol on ethylene diamine hydrochloride, or by reduction of pyrazine with sodium in ethanol.HPLC of Formula: 224785-90-4
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics