Sawada, Minoru et al. published their research in Bunseki Kagaku in 1986 | CAS: 316-81-4

N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4) belongs to piperazine derivatives. Industrial applications of piperazine include the manufacture of plastics, resins, pesticides and brake fluids. Piperazine and its salts did not induce point mutations in a bacterial test. A series of mutagenicity studies in cells, both in vitro and in vivo, has been completed and showed no evidence of mutagenic effect.COA of Formula: C22H30N4O2S2

Automated determination of phenothiazine drugs by spectrophotometry with palladium(II) chloride was written by Sawada, Minoru;Motoyama, Ichiro;Yamada, Sachiko;Kanaya, Yoshikiyo. And the article was included in Bunseki Kagaku in 1986.COA of Formula: C22H30N4O2S2 This article mentions the following:

An automated assay for phenothiazine drugs using a PdCl2 solution was established by application of a continuous flow anal. A sample was dissolved in a mixture of 0.625M H2SO4 and DMF (2:3) to make about 3 × 10-3M solution A standard solution was prepared in the same way. A 4.2 × 10-3M PdCl2 stock solution was prepared by dissolving PdCl2 in 0.625M H2S4 and diluted with DMF (4:1) before use. Using an automatic analyzer, the sample solution was diluted with a mixture of 0.625M H2SO4 and DMF (4:1) to one-seventh of the concentration, and the diluted sample solution was added to a 6-fold volume of the PdCl2 solution The absorbance of this mixture was determined at 490 nm. The accuracy was 99-100% except pharmaceutical preparations containing excess chloride and the reproducibility was 0.1-0.3%. It was possible to assay up to 30 samples per day which are 2- to 3-fold more than those handled manually. The continuous variation methods showed that the Pd complex was a 1:1 of Pd-phenothiazine. The absorption at about 490 nm resulting from the Pd complex was affected by substituents at 2- and 10-positions of a phenothiazine ring. In the experiment, the researchers used many compounds, for example, N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4COA of Formula: C22H30N4O2S2).

N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4) belongs to piperazine derivatives. Industrial applications of piperazine include the manufacture of plastics, resins, pesticides and brake fluids. Piperazine and its salts did not induce point mutations in a bacterial test. A series of mutagenicity studies in cells, both in vitro and in vivo, has been completed and showed no evidence of mutagenic effect.COA of Formula: C22H30N4O2S2

Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics