Into the first biomimetic sphingomyelin stationary phase: Suitability in drugsprime biopharmaceutic profiling and block relevance analysis of selectivity was written by Russo, Giacomo;Ermondi, Giuseppe;Caron, Giulia;Verzele, Dieter;Lynen, Frederic. And the article was included in European Journal of Pharmaceutical Sciences in 2021.COA of Formula: C32H39NO4 The following contents are mentioned in the article:
Sphingomyelin (SPH) is a type of sphingolipid found in animal nerve tissues, especially in the membranous myelin sheath that surrounds some nerve cell axons. Because of its characteristics, SPH stationary phase represents an ideal tool to mimic the interactions taking place between active pharmaceutical ingredients and neurons.The IAM. SPH stationary phase (0.821 mg) was suspended in methanol (7.0 mL) and the resulting slurry packed (600 bar) in an HPLC column (10 cm x 2.1 mm). The column was operated at 300 muL min-1 at 25degC using a mobile phase consisting of 60/25/15 (volume/volume/v) Dulbeccoprimes phosphate buffer saline pH 7.4/methanol/acetonitrile. The elution was achieved isocratically and monitored by UV detection at 220 nm. The investigated dataset consisted of 88 compounds (36 neutrals, 26 bases and 26 acids). The block relevance (BR) anal. was accomplished starting by calculating 82 descriptors using the software VS+ and submitting the data matrixes to Matlab. Multiple linear regression and related descriptors were obtained with Vega ZZ 64. The method developed allowed to achieve a solid and reproducible SPH affinity scale for the assayed compounds Computational studies produced statistically significant models for the prediction and mechanism elucidation of the retentive behavior of pharmaceutically relevant compounds on the SPH stationary phase. For ionizable compounds, the IAM. SPH exhibited an original selectivity when compared to the com. available IAM.PC. Moreover, apart from its suitability to surrogate log BB, IAM. SPH was also found relate significantly with the drugsprime fraction unbound in plasma, a crucial parameter in pharmacokinetics. This study involved multiple reactions and reactants, such as 2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid (cas: 83799-24-0COA of Formula: C32H39NO4).
2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid (cas: 83799-24-0) belongs to piperidine derivatives. The piperidine structural motif is present in numerous natural alkaloids. These include piperine, which gives black pepper its spicy taste. Several piperidine alkaloids isolated from natural herbs, were found to exhibit antiproliferation and antimetastatic effects on various types of cancers both in vitro and in vivo for example Piperine, Evodiamine, Matrine, Berberine and Tetrandine.COA of Formula: C32H39NO4
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem