Comparative study on retention behaviour and enantioresolution of basic and neutral structurally unrelated compounds with cellulose-based chiral stationary phases in reversed phase liquid chromatography-mass spectrometry conditions was written by Perez-Baeza, Mireia;Escuder-Gilabert, Laura;Martin-Biosca, Yolanda;Sagrado, Salvador;Medina-Hernandez, Maria Jose. And the article was included in Journal of Chromatography A in 2022.Category: piperidines This article mentions the following:
A comparative study on the retention behavior and enantioresoln. of 54 structurally unrelated neutral and basic compounds using five com. cellulose-based chiral stationary phases (CSPs) and hydro-organic mobile phases compatible with MS detection is performed. Four phenylcarbamate-type cellulose CSPs (cellulose tris(3,5-dimethylphenylcarbamate), Cell1; cellulose tris(3-chloro-4-methylphenylcarbamate), Cell2; cellulose tris(4-chloro-3-methylphenylcarbamate), Cell4 and cellulose tris(3,5- dichlorophenylcarbamate), Cell5) and one benzoate-type cellulose CSP (cellulose tris(4-methylbenzoate), Cell3) are assayed. Mobile phases consist of binary mixtures of methanol (30-90% MeOH) or acetonitrile (10-98% ACN) with 5 mM ammonium bicarbonate (pH = 8.0). The existence of reversed phase (RPLC) and hydrophilic interaction liquid chromatog. (HILIC) retention behavior domains is explored. In MeOH/H2O mobile phases, for all compounds and CSPs, the typical RPLC retention behavior is observed When using ACN/H2O mobile phases, for all compounds in all CSPs (even in the non-chlorinated CSPs) a U-shaped retention behavior depending on the ACN/H2O content is observed which indicates the coexistence of the RPLC- (< 80% ACN) and HILIC- (鈭?0-98% ACN) domains. The magnitude of retention changes in both domains is related to the hydrophobicity of the compound as well as to the nature of the CSP. The study of the effect of the nature and concentration of the organic solvent, as well as the nature of the CSP on the enantioresoln. reveals that: (i) the use of MeOH/H2O or ACN/H2O greatly affects the enantioselectivity and enantioresoln. degree of the chromatog. systems, being, in general, better the results obtained with ACN/H2O mobile phases. (ii) The ACN-RPLC-domain provides much better enantioresoln. than HILIC-domain. (iii) Cell2, especially with ACN/H2O mobile phases, is the CSP that allows baseline enantioresoln. for a higher number of compounds (iv) Phenylcarbamate-type CSPs do not offer clear complementary enantioselectivity to that of Cell2. (v) Cell3 is the only CSP that provides marked complementary enantioselectivity to that of Cell2, almost orthogonal in MeOH/H2O mobile phases. In the experiment, the researchers used many compounds, for example, 10-(2-(1-Methylpiperidin-2-yl)ethyl)-2-(methylthio)-10H-phenothiazine (cas: 50-52-2Category: piperidines).
10-(2-(1-Methylpiperidin-2-yl)ethyl)-2-(methylthio)-10H-phenothiazine (cas: 50-52-2) belongs to piperidine derivatives. Piperidine is a saturated organic heteromonocyclic parent, an azacycloalkane, a secondary amine and a member of piperidines. Fluorinated piperidines are also the subject of continued interest in medicinal chemistry, for example in the synthesis of selective dipeptidyl peptidase II (DPP II) inhibitors. Piperidine derivatives are also used in solid-phase peptide synthesis (SPPS) and many degradation reactions.Category: piperidines
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem