Application of 143900-44-1, Enzymes are biological catalysts that produce large increases in reaction rates and tend to be specific for certain reactants and products. 143900-44-1, Name is (S)-tert-Butyl 3-hydroxypiperidine-1-carboxylate, SMILES is O=C(N1C[C@@H](O)CCC1)OC(C)(C)C, belongs to piperidines compound. In a article, author is Cioroiu, Bogdan I., introduce new discover of the category.
New approach for determination of the degradation products of fenspiride hydrochloride found in oral liquid formulations
Fenspiride hydrochloride (FNS) is used in treating chronic inflammatory diseases, most commonly as a liquid oral solution. FNS produces degradation products along with fenspiride N-oxide (FNO) and 1-phenylethyl-4-hydroxy-4-aminomethyl piperidine hydrochloride (PHAP). We aimed to develop and validate a chromatographic method in order to identify the main degradation products in the presence of other compounds from a liquid preparation. The method used a dual gradient using two buffer solutions: the first with pH4.5 (buffer 1, pH4.5-MeOH 90:10%, v/v) and the second with pH2.9 (buffer 2, pH2.9-acetronitrile-methanol, 65:15:10%, v/v/v). As mentioned, there was a modification of the organic mixture, starting with 10% methanol and ending with a mixture of acetonitrile-methanol (15:10%, v/v). The flow-rate was 1.5mL/min. According to the elution program, experimental conditions started with 100% solution S1, which decreased to 0% and, simultaneously, solution S2 increased to 100% during the first 10min and was maintained for a further 5min. After 15min, initial conditions were re-established. The linearity interval was 0.5-2g/mL and the minimum correlation coefficient was 0.999. The recovery factor was 100.47-103.17% and the limit of quantification was 0.19-0.332g/mL. Intra-day maximum precision was 4.08% for FNS and 2.65% for PHAP. This double-gradient mobile phase produced good specificity in relation to the degradation products of FNS and other constituents of the oral liquid formulation. Forced degradation studies revealed other related substances that were confirmed in mass balance analyses. Degradation products were confirmed in acidic, basic and oxidative media.
Application of 143900-44-1, Because enzymes can increase reaction rates by enormous factors and tend to be very specific, typically producing only a single product in quantitative yield, they are the focus of active research.you can also check out more blogs about 143900-44-1.
Reference:
Piperidine – Wikipedia,
,Piperidine | C5H11N – PubChem