Thin-layer chromatography gradient optimization strategy for wet load adsorption flash chromatography was written by Krecisz, Pawel;Czarnecka, Kamila;Szymanski, Pawel. And the article was included in Journal of Chromatographic Science in 2022.COA of Formula: C21H26N2S2 This article mentions the following:
Chromatog. is one of the most popular methods for the separation of compounds in modern pharmaceutical industry and science. Despite the extensive use of the reversed phase chromatog. in anal. and preparative applications, the normal phase adsorption chromatog. has a special place in purifying post-reaction mixtures or the separation of natural extracts, especially in wet load mode, because of simplicity and high velocity of preparation Complex mixtures, more difficult to sep., require gradient methods to obtain better results of separations These methods can be developed by external software, but the automatic methods are often not very accurate and the neg. impact of wet load application on separation quality is considerable in them. Therefore, we present the thin-layer chromatog. (TLC) gradient optimization strategy for wet load separations to obtain repeatable results of separations for different compounds without worrying about neg. impact of wet loading on separation quality. The strategy provides information about an elution model of desired compound, which is used to develop the gradient method. The strategy also allows to standardize the separation length, because gradient methods performed by the TLC gradient optimization strategy have a very similar duration time in column volumes The method can also be simply scaled because of using the column volume as a base unit in calculations In the experiment, the researchers used many compounds, for example, 10-(2-(1-Methylpiperidin-2-yl)ethyl)-2-(methylthio)-10H-phenothiazine (cas: 50-52-2COA of Formula: C21H26N2S2).
10-(2-(1-Methylpiperidin-2-yl)ethyl)-2-(methylthio)-10H-phenothiazine (cas: 50-52-2) belongs to piperidine derivatives. The piperidine ring can be found not only in more than half of the currently known structures of alkaloids, but also in many natural or synthetic compounds with interesting biological activities. Several piperidine alkaloids isolated from natural herbs, were found to exhibit antiproliferation and antimetastatic effects on various types of cancers both in vitro and in vivo for example Piperine, Evodiamine, Matrine, Berberine and Tetrandine.COA of Formula: C21H26N2S2
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem