Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label was written by Heaven, Graham;Hollas, Michael A.;Tabernero, Lydia;Fielding, Alistair J.. And the article was included in Applied Magnetic Resonance in 2021.Application of 2896-70-0 This article mentions the following:
Structural investigations of proteins and their biol. complexes are now frequently complemented by distance constraints between spin labeled cysteines generated using double electron-electron resonance (DEER) spectroscopy, via site directed spin labeling (SDSL). Methanethiosulfonate spin label (MTSSL), has become ubiquitous in the SDSL of proteins, however, has limitations owing to its high number of rotamers, and reducibility. In this article we introduce the use of bromoacrylaldehyde spin label (BASL) as a cysteine spin label, demonstrating an advantage over MTSSL due to its increased selectivity for surface cysteines, eliminating the need to ‘knock out’ superfluous cysteine residues. Applied to the multidomain protein, His domain protein tyrosine phosphatase (HD-PTP), we show that BASL can be easily added in excess with selective labeling, whereas MTSSL causes protein precipitation Furthermore, using DEER, we were able to measure a single cysteine pair distance in a three cysteine domain within HD-PTP. The label has a further advantage of comprising a sulfide in a three-bond tether, making it a candidate for protein binding and in-cell studies. In the experiment, the researchers used many compounds, for example, 4-Oxo-tempo (cas: 2896-70-0Application of 2896-70-0).
4-Oxo-tempo (cas: 2896-70-0) belongs to piperidine derivatives.Piperidine is a key saturated heterocyclic scaffold found in several of the top-selling small molecule pharmaceuticals and natural alkaloids, with a diverse range of biological activities. Some chemotherapeutic agents have piperidine moiety within their structure, foremost among them, vinblastine and raloxifene.Application of 2896-70-0
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem