Eissler, Stefan published the artcileSubstitution determination of Fmoc-substituted resins at different wavelengths, Computed Properties of 35661-58-6, the publication is Journal of Peptide Science (2017), 23(10), 757-762, database is CAplus and MEDLINE.
In solid-phase peptide synthesis, the nominal batch size is calculated using the starting resin substitution and the mass of the starting resin. The starting resin substitution constitutes the basis for the calculation of a whole set of important process parameters, such as the number of amino acid derivative equivalent For Fmoc-substituted (Fmoc = 9-fluorenyl-methoxycarbonyl) resins, substitution determination is often performed by suspending the Fmoc-protected starting resin in 20% (volume/volume) piperidine in DMF to generate the dibenzofulvene-piperidine adduct that is quantified by UV-visible spectroscopy. The spectrometric measurement is performed at the maximum absorption wavelength of the dibenzofulvene-piperidine adduct, i.e., at 301.0 nm. The recorded absorption value, the resin weight and the volume are entered into an equation derived from Lambert-Beer’s law, together with the substance-specific molar absorption coefficient at 301.0 nm, in order to calculate the nominal substitution. To our knowledge, molar absorption coefficients between 7100 l mol-1 cm-1 and 8100 l mol-1 cm-1 have been reported for the dibenzofulvene-piperidine adduct at 301.0 nm. Depending on the applied value, the nominal batch size may differ up to 14%. In this publication, a determination of the molar absorption coefficients at 301.0 and 289.8 nm is reported. Furthermore, proof is given that by measuring the absorption at 289.8 nm the impact of wavelength accuracy is reduced. StartCopText© 2017 The Authors Journal of Peptide Science published by European Peptide Society and John Wiley & Sons Ltd.
Journal of Peptide Science published new progress about 35661-58-6. 35661-58-6 belongs to piperidines, auxiliary class Piperidine,Benzene, name is 1-((9H-Fluoren-9-yl)methyl)piperidine, and the molecular formula is C19H21N, Computed Properties of 35661-58-6.
Referemce:
https://en.wikipedia.org/wiki/Piperidine,
Piperidine | C5H11N – PubChem