In 2018,Demuth, Jiri; Kucera, Radim; Kopecky, Kamil; Havlinova, Zuzana; Libra, Antonin; Novakova, Veronika; Miletin, Miroslav; Zimcik, Petr published 《Efficient synthesis of a wide-range absorbing azaphthalocyanine dark quencher and its application to dual-labeled oligonucleotide probes for quantitative real-time polymerase chain reactions》.Chemistry – A European Journal published the findings.Category: piperidines The information in the text is summarized as follows:
Unsym. dialkylamino-substituted zinc azaphthalocyanine (AzaPc) exhibits unique spectral and photophys. properties for dark quenchers of fluorescence in DNA hybridization probes. The panchromatic light absorption of AzaPc from 300 nm up to at least 700 nm and its lack of fluorescence make it an ideal candidate for a universal dark quencher. To prove this exptl., oligodeoxyribonucleotide probes were labeled at the 3′-end by this AzaPc and at the 5′-end by a fluorophore used in the polymerase chain reaction (PCR)-i.e., fluorescein, CAL Fluor Red 610, and Cy5. AzaPc showed a significantly higher quenching efficiency compared to the com. available dark quenchers (BHQ-1, BHQ-2, BBQ-650) in a developed model of TaqMan PCR assay. The AzaPc-labeled probe proved to also be useful in a practical PCR assay for the quantification of the SLCO2B1 transporter gene expression. The constructed calibration curves indicated linearity in the range from 102 to 107 of target copies. After reading the article, we found that the author used 2-(Piperidin-4-yl)ethanol(cas: 622-26-4Category: piperidines)
2-(Piperidin-4-yl)ethanol(cas: 622-26-4) can be used to synthese ursolic acid derivatives, spiroimidazolidinone NPC1L1 inhibitors, neurokinin-2 receptor antagonists, antagonists for inhibition of platelet aggregation.Category: piperidines
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem