A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate was written by Bircsak, Kristin M.;DeBiasio, Richard;Miedel, Mark;Alsebahi, Alaa;Reddinger, Ryan;Saleh, Anthony;Shun, Tongying;Vernetti, Lawrence A.;Gough, Albert. And the article was included in Toxicology in 2021.Quality Control of 2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid The following contents are mentioned in the article:
We report development, automation and validation of 3D, microfluidic liver-on-a-chip for high throughput hepatotoxicity screening, OrganoPlate LiverTox. The model is comprised of aggregates of induced pluripotent stem cell-derived hepatocytes seeded in an extracellular matrix in organ channel and co-cultured with endothelial cells and THP-1 monoblasts differentiated to macrophages seeded in vascular channel of the 96 well Mimetas OrganoPlate 2-lane. A combination of secretome measurements and image-based anal. was used to demonstrate stable 15 day cell viability, albumin and urea secretion. Over same time-period, CYP3A4 activity increased and alpha-fetoprotein secretion decreased suggesting further maturation of iHeps. Troglitazone, clin. hepatotoxin, was chosen as control compound for validation studies. Albumin, urea, hepatocyte nuclear size and viability staining provided Robust Z’factors > 0.2 in plates treated 72 h with 180μM troglitazone compared with vehicle control. The viability assay provided the most robust statistic for a Robust Z’ factor = 0.6. A small library of 159 compounds with known liver effects was added to OrganoPlate LiverTox model for 72 h at 50μM and Toxicol. Prioritization scores were calculated A follow up dose-response evaluation of select hits revealed albumin assay to be most sensitive in calculating TC50 values. This platform provides a robust, novel model which can be used for high throughput hepatotoxicity screening. This study involved multiple reactions and reactants, such as 2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid (cas: 83799-24-0Quality Control of 2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid).
2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid (cas: 83799-24-0) belongs to piperidine derivatives. Piperidine has a role as a reagent, a protic solvent, a base, a catalyst, a plant metabolite, a human metabolite and a non-polar solvent. Industrially, piperidine is produced by the hydrogenation of pyridine, usually over a molybdenum disulfide catalyst. Pyridine can also be reduced to piperidine via a modified Birch reduction using sodium in ethanol.Quality Control of 2-(4-(1-Hydroxy-4-(4-(hydroxydiphenylmethyl)piperidin-1-yl)butyl)phenyl)-2-methylpropanoic acid
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem