Determination of ropivacaine and its metabolites in human plasma using solid phase microextraction and GC-NPD / GC-MS was written by Abdel-Rehim, Mohamed;Andersson, Mikael;Portelius, Erik;Norsten-Hoog, Carina;Blomberg, Lars G.. And the article was included in Journal of Microcolumn Separations in 2001.Application of 27262-40-4 This article mentions the following:
The performance of solid-phase microextraction (SPME) in combination with capillary gas chromatog. (CGC) to quantify ropivacaine and its metabolites in human plasma was studied. The anal. was performed using either a N P detector (NPD) or a mass-spectrometric detector. For extraction, Carbowax / divinylbenzene, polyacrylate and polydimethylsiloxane fibers were tested. Absorption and desorption times were studied for all analytes sep. The Carbowax / divinylbenzene fiber gave the highest recovery in plasma samples as compared to the other fibers. The effects of temperature, addition of salt, and agitation of the sample were studied. The validation of the method showed that the chromatog. selectivity was satisfactory and all metabolites were well separated SPME gave higher deviation as compared to published data for solid-phase and liquid-liquid extraction as sample preparation methods but the acceptance criteria for the study validation were well in line with the international criteria. The major disadvantage of SPME in quant. bioanal. is that the fiber does not withstand a complete run (standards + blanks + QC samples + patient samples). Also, the quality of fiber and the fiber length can differ from batch to batch. In the experiment, the researchers used many compounds, for example, (S)-N-(2,6-Dimethylphenyl)-2-piperidinecarboxamide (cas: 27262-40-4Application of 27262-40-4).
(S)-N-(2,6-Dimethylphenyl)-2-piperidinecarboxamide (cas: 27262-40-4) belongs to piperidine derivatives. Piperidine has a role as a reagent, a protic solvent, a base, a catalyst, a plant metabolite, a human metabolite and a non-polar solvent. Industrially, piperidine is produced by the hydrogenation of pyridine, usually over a molybdenum disulfide catalyst. Pyridine can also be reduced to piperidine via a modified Birch reduction using sodium in ethanol.Application of 27262-40-4
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem