Simple and simultaneous determination for 12 phenothiazines in human serum by reversed-phase high-performance liquid chromatography was written by Tanaka, Einosuke;Nakamura, Takako;Terada, Masaru;Shinozuka, Tatsuo;Hashimoto, Chikako;Kurihara, Katsuyoshi;Honda, Katsuya. And the article was included in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences in 2007.Application In Synthesis of N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide This article mentions the following:
A high-performance liquid chromatog. method has been developed for the simultaneous anal. of the 12 phenothiazines (chlorpromazine, fluphenazine, levomepromazine, perazine, perphenazine, prochlorperazine, profenamine, promethazine, propericiazine, thioproperazine, thioridazine and trifluoperazine) in human serum using HPLC/UV. The separation was achieved using a C18 reversed-phase column (250 mm × 4.6 mm I.D., particle size 5 μm, Inersil ODS-SP). The mobile phase, consisting of acetonitrile-methanol-30 mM NaH2PO4 (pH 5.6) (300:200:500, volume/volume/v), was delivered at a flow rate of 0.9 mL/min and UV detection was carried out at 250 nm. The recoveries of the 12 phenothiazines spiked into serum samples were 87.6-99.8%. Regression equations for the 12 phenothiazines showed excellent linearity, with detection limits of 3.2-5.5 ng/mL for serum. The inter-day and intra-day coefficients of variation for serum samples were commonly below 8.8%. The selectivity, accuracy and precision of this method are satisfactory for clin. and forensic purposes. This sensitive and selective method offers the opportunity for simultaneous screening and quantification of almost all phenothiazines available in Japan for the purposes of clin. and forensic applications. In the experiment, the researchers used many compounds, for example, N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4Application In Synthesis of N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide).
N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4) belongs to piperazine derivatives. Piperazine belongs to the family of medicines called anthelmintics. Piperazine is formed as a co-product in the ammoniation of 1,2-dichloroethane or ethanolamine. These are the only routes to the chemical used commercially.Application In Synthesis of N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics