MALDI-TOF mass spectrometric determination of 11 phenothiazines with heavy side chains in urine was written by Minakata, Kayoko;Yamagishi, Itaru;Nozawa, Hideki;Gonmori, Kunio;Hasegawa, Koutaro;Wurita, Amin;Suzuki, Masako;Watanabe, Kanako;Suzuki, Osamu. And the article was included in Forensic Toxicology in 2013.SDS of cas: 316-81-4 This article mentions the following:
A rapid screening method was developed for the determination of phenothiazines by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In this method, α-cyano-4-hydroxy cinnamic acid (CHCA) was used as the matrix to assist the ionization of phenothiazines. The identification of 11 phenothiazines with heavy side chains was achieved by observing their protonated mol. ions [M+H]+ at m/z 340-447. Quantification was achieved by using triflupromazine at m/z 353 as the internal standard (IS). The relative ionization efficiencies of 11 phenothiazines and IS on MALDI-TOF-MS were different from those achieved by ESI-MS, but the product ion spectra from MALDI-MS-MS were quite similar to those from ESI-MS-MS except in the case of flupentixol. The limit of detection was 0.3 ng/mL with a quantification range of 1-50 ng/mL urine in the best case; the limit of detection was 5 ng/mL with a quantification range of 10-100 ng/mL urine in the worst case for 10 phenothiazines except thiethylperazine. The present method provides a simple and high-throughput method for the screening of these phenothiazines using only 20 μl of urine. To our knowledge, this study is the first trial to analyze phenothiazines by MALDI-TOF-MS (-MS). In the experiment, the researchers used many compounds, for example, N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4SDS of cas: 316-81-4).
N,N-Dimethyl-10-(3-(4-methylpiperazin-1-yl)propyl)-10H-phenothiazine-2-sulfonamide (cas: 316-81-4) belongs to piperazine derivatives. Piperazine causes primary dermal irritation and skin burns at high concentrations. Piperazine also causes eye irritation in humans. Outside the body, piperazine has a remarkable power to dissolve uric acid and producing a soluble urate, but in clinical experience it has not proved equally successful. SDS of cas: 316-81-4
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics