Influence of uncharged mobile phase additives on retention and enantioselectivity of chiral drugs using an 伪1-acid glycoprotein column was written by Enquist, Muerit;Hermansson, Joergen. And the article was included in Journal of Chromatography in 1990.Computed Properties of C14H20N2O This article mentions the following:
The effect of uncharged mobile phase additives on retention and enantioselectivity on a chiral 伪1-acid glycoprotein (AGP) column was investigated. Chiral selectivity for several drugs can be induced by adding to the mobile phase uncharged modifiers with different hydrophobicities and different hydrogen bonding properties. Modifiers with different hydrogen bonding properties affect the enantioselectivity in different ways. The solute enantiomers seem to compete with the modifier mols. for binding to the chiral stationary phase. The adsorption of PrOH and MeCN on the AGP column was measured. A monolayer was obtained at mobile phase concentrations of 1.3M (10%) and 2.8M (15%) for AcOH and MeCN, resp. These concentrations are in the ranges usually used for chromatog. studies. The effect of 2-propanol on the protein conformation was studied using CD spectroscopy. It was not possible to detect any changes in the conformation of AGP, even in the presence of 40% 2-propanol. In the experiment, the researchers used many compounds, for example, (S)-N-(2,6-Dimethylphenyl)-2-piperidinecarboxamide (cas: 27262-40-4Computed Properties of C14H20N2O).
(S)-N-(2,6-Dimethylphenyl)-2-piperidinecarboxamide (cas: 27262-40-4) belongs to piperidine derivatives. Piperidine has a role as a reagent, a protic solvent, a base, a catalyst, a plant metabolite, a human metabolite and a non-polar solvent. The piperidine and polyhydroxylated indolizidine derivatives have shown to be promising 伪-glucosidase inhibitors. The former are analogs of DNJ with an improved 伪-glucosidase inhibitory profile than that of DNJ. Boisson et al.Computed Properties of C14H20N2O
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem