Monensin biodegradation pathway and role of epoxide hydrolase in Stenotrophomonas maltophilia DM-2 was written by Li, Hao;Li, Jie;Wan, Qiang;Wang, Chuanwen;Sun, Weiwei;Zhao, Jiayi;Xing, Yidan;Pan, Baoliang. And the article was included in Journal of Chemical Technology and Biotechnology in 2020.Product Details of 1222780-33-7 The following contents are mentioned in the article:
Monensin is widely used in livestock and poultry production to promote animal growth and control coccidiosis. Most monensin is excreted as unchanged parent compounds via feces. However monensin is very stable and difficult to degrade in the environment, and is potentially risky for the health of wildlife and humans. In this study, we found that a Stenotrophomonas maltophilia DM-2 strain isolated from chicken manure could effectively degrade monensin. The optimum temperature and pH for DM-2 to degrade monensin were 40 °C and pH 7.0. A potential degradation pathway of monensin was proposed based on the identification and characterization of two new monensin metabolic intermediates by liquid chromatog. quadrupole time-of-flight mass spectroscopy. In addition, an inducible monensin-degrading activity was present in DM-2. A soluble epoxide hydrolysis (sEH) gene highly expressed in the DM-2 on day 3 and 6 under monensin stress, which was not observed in the DM-2 without monensin stress. When a specific inhibitor TPPU (1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy)phenyl]urea) of sEH was added into the cell-free extract, the degradation of monensin was inhibited. Strain DM-2 can biodegrade monensin by soluble epoxy hydrolase and produce two new intermediates. To our knowledge, this is the first report of the degradation of monensin by Stenotrophomonas strains. The novel metabolic pathways of monensin which we found may provide new insight into the environmental fate of monensin. This study involved multiple reactions and reactants, such as 1-(1-Propionylpiperidin-4-yl)-3-(4-(trifluoromethoxy)phenyl)urea (cas: 1222780-33-7Product Details of 1222780-33-7).
1-(1-Propionylpiperidin-4-yl)-3-(4-(trifluoromethoxy)phenyl)urea (cas: 1222780-33-7) belongs to piperidine derivatives. Piperidine is a metabolite of cadaverine, a polyamine found in the human intestine. Several piperidine alkaloids isolated from natural herbs, were found to exhibit antiproliferation and antimetastatic effects on various types of cancers both in vitro and in vivo for example Piperine, Evodiamine, Matrine, Berberine and Tetrandine.Product Details of 1222780-33-7
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem