On April 30, 2018, Mieszkowski, Dominik; Sroka, Wiktor Dariusz; Marszall, Michat Piotr published an article.Category: piperidines The title of the article was Ionic liquids as separation enhancers of haloperidol and its two metabolites in high-performance thin-layer chromatography supported with mass spectrometry. And the article contained the following:
High-performance thin-layer chromatog. (HPTLC)-densitometric method of haloperidol (HP) and its two metabolites (reduced haloperidol [RHP], 4-(4-chlorophenyl)-4-hydroxypiperidine [CPHP]) from human plasma has been developed by use of mobile-phase additives. The influence of the type of inorganic/organic additive on the retention of the studied compounds was evaluated. The chromatog. process was carried out with traditional mobile phase modifiers and 1-alkyl-imidazolium ionic liquid as separation enhancers, in the presence of chlorpromazine as internal standard 1-Ethyl-3-methylimidazolium tetrafluoroborate ([emim][BF4]) ionic liquid offered good selectivity in comparison with traditional mobile phase additives. The studied drugs were well distributed as the RF values were 0.31 for chlorpromazine hydrochloride (CPZ), 0.38 for HP, 0.44 for CPHP, and 0.58 for RHP, resp., with no apparent broadening and overlapping of spots. The test compounds were extracted using acetonitrile as precipitation agent. The identity of the bands from human plasma was addnl. confirmed by rapid and contamination-free CAMAG thin-layer chromatog.-mass spectrometry (TLC-MS) interface. The limit of detection (LOD) values obtained by densitometry scanning were 0.1807, 0.3158, and 0.3924μg spot-1 (for HP, RHP, and CPHP), whereas the limit of quantification (LOQ) values for the presented method were 0.5476, 0.9570, and 1.1892μg spot-1 (for HP, RHP, and CPHP). Recovery values of all tested compounds were in the range from 95.43% to 99.60% (intra-day) and 96.13% to 103.18% (inter-day);%RSD did not exceed the value of 5%. The results confirm the pos. effect of ionic liquids in the separation process related to their silanol blocking properties and their suitability for use in thin-layer chromatog./mass spectrometry method. The experimental process involved the reaction of 4-(4-Chlorophenyl)piperidin-4-ol(cas: 39512-49-7).Category: piperidines
The Article related to ionic liquid haloperidol metabolite thin layer chromatog mass spectrometry, Organic Analytical Chemistry: Other and other aspects.Category: piperidines
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem