Kumar, Aditya; Prasad, Ankush; Sedlarova, Michaela; Pospisil, Pavel published an article on January 31 ,2019. The article was titled 《Organic radical imaging in plants: Focus on protein radicals》, and you may find the article in Free Radical Biology & Medicine.Formula: C9H17NO The information in the text is summarized as follows:
Biomol. (lipid and protein) oxidation products formed in plant cells exposed to photooxidative stress play a crucial role in the retrograde signaling and oxidative damage. The oxidation of biomols. initiated by reactive oxygen species is associated with formation of organic (alkyl, peroxyl and alkoxyl) radicals. Currently, there is no selective and sensitive technique available for the detection of organic radicals in plant cells. Here, based on the analogy with animal cells, immuno-spin trapping using spin trap, 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was used to image organic radicals in Arabidopsis leaves exposed to high light. Using antibody raised against the DMPO nitrone adduct conjugated with the fluorescein isothiocyanate, organic radicals were imaged by confocal laser scanning microscopy. Organic radicals are formed predominantly in the chloroplasts located at the periphery of the cells and distributed uniformly throughout the grana stack. Characterization of protein radicals by standard immunol. techniques using anti-DMPO antibody shows protein bands with apparent mol. weights of 32 and 34 kDa assigned to D1 and D2 proteins and two protein bands below the D1/D2 band with apparent mol. weights of 23 and 18 kDa and four protein bands above the D1/D2 band with apparent mol. weights of 41, 43, 55 and 68 kDa. In summary, imaging of organic radicals by immuno-spin trapping represents selective and sensitive technique for the detection of organic radicals that might help to clarify mechanistic aspects on the role of organic radicals in the retrograde signaling and oxidative damage in plant cell. The experimental part of the paper was very detailed, including the reaction process of Triacetonamine(cas: 826-36-8Formula: C9H17NO)
Triacetonamine(cas: 826-36-8) is a member of piperidine. Piperidine-containing compounds are also frequently employed in synthesis as ligands or auxiliaries. Accordingly, many efforts have been devoted to the development of novel methods for the synthesis of these compounds over the years.Formula: C9H17NO
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem