Cheng, Yarui; Zhang, Tianyuan; Cao, Yangrong; Wang, Li; Chen, Wenli published an article in Applied Microbiology and Biotechnology. The title of the article was 《New insights into the function of the proteins IsiC and IsiD from Synechocystis sp. PCC 6803 under iron limitation》.COA of Formula: C6H12N2O The author mentioned the following in the article:
Iron is a common cofactor in biol. processes such as respiration, photosynthesis, and nitrogen fixation. The genes isiC and isiD encode unknown proteins, and the growth of ΔisiC and ΔisiD mutants is inhibited under iron-deficient conditions. To study the regulatory mechanisms of IsiC and IsiD during iron starvation, we carried out transcriptome and metabolome sequencing. The Kyoto Encyclopedia of Genes and Genomes (KEGG) anal. showed that the photosynthesis, nitrogen metabolism, and ABC transporter pathways play a vital role in regulating iron deficiency. Upon iron repletion, IsiC and IsiD also have a regulatory effect on these pathways. Addnl., KEGG anal. of the differential metabolites of wild type (WT) and mutants showed that they were all enriched in starch and sucrose metabolism after iron limitation. Weighted gene co-expression network anal. (WGCNA) constructed a co-expression network of differentially expressed genes with phenotypes and metabolites, and finally identified five modules. The turquoise module was pos. correlated with iron deficiency. In contrast, the WT and blue module exhibited a neg. correlation, and the mutants ΔisiC and ΔisiD were pos. correlated with the gray and brown modules, resp. WGCNA also analyzed the relationship between metabolites and phenotypes, and the green module was related to iron starvation. The co-expression network determined the hub genes and metabolites of each module. This study lays a foundation for a better understanding of cyanobacteria in response to iron deficiency. In addition to this study using Piperidine-4-carboxamide, there are many other studies that have used Piperidine-4-carboxamide(cas: 39546-32-2COA of Formula: C6H12N2O) was used in this study.
Piperidine-4-carboxamide(cas: 39546-32-2) belongs to anime. The reaction of alkyl halides, R―X, where X is a halogen, or analogous reagents with ammonia (or amines) is useful with certain compounds. Not all alkyl halides are effective reagents; the reaction is sluggish with secondary alkyl groups and fails with tertiary ones. Its usefulness is largely confined to primary alkyl halides (those having two hydrogen atoms on the reacting site).COA of Formula: C6H12N2O
Referemce:
Piperidine – Wikipedia,
Piperidine | C5H11N – PubChem